PFA fixed cells

Aleta Knowles (A.Knowles@vetp.usyd.edu.au)
Fri, 2 Aug 1996 10:57:59 +1100

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Hello everyone,
I'm looking at phagocytosis and oxidative burst in porcine neutrophils.
I plan on performing a simultaneous assay using FITC-zymosan and
hydroethidium, and will be processing lots of samples by flow cytometry.
I would like to fix the cells in PFA after phagocytosis - will fixation
affect the resulting flourescence of EtBr produced during the oxidative
burst?
Thanks
Aleta

****************************************************************************** Aleta Knowles

Department of Veterinary Pathology Phone: 61 2 351 7651 The University of Sydney Fax: 61 2 351 7348 NSW, 2006 AUSTRALIA

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu