RE: Dyes for cell tracking experiments...
Dan Smith (DJS@ALLP.COM)
Tue, 23 Jul 96 16:24:00 PDT
We have used a label from Zynaxis (now carried by Sigma) called PKH-26. It
intercalates into the phospholipid membrane of the cell and is probably
stable for the life of the cell. If the cell is mitotic, it will divide
with it and the fluorescence will also half. It will excite at 488nm and
emit in the 570nm range I believe.
Good luck,
Dan J. Smith
San Diego, CA
----------
{From: owner-cyto-sendout
{To: Cytometry Mailing List
{Subject: Dyes for cell tracking experiments...
{Date: Tuesday, July 23, 1996 4:54PM
{
{
{
{Greetings Flow-ers,
{
{
{We intend to perform an experiment which will involve the labelling of
{isolated mouse tumour cells with a fluorescent dye, prior to introducing
{these cells into a mixed population. The idea is to be able to detect these
{original cells in the mixture by flow, after a period of time lasting
around
{two hours. So, the question is this: which dye would any of you esteemed
flow
{gurus recommend for this, which will:-
{
{ Readily be taken up by viable cells.
{ Not be removed by washing immediately afterwards.
{ Persist for at least two hours, without serious loss of
fluorescence.
{
{ Potential excitation wavelength for the dye to be used is not a
{problem, as the analysis will be performed using a FACStar+ with a choice
of
{488nm., UV & dye laser excitation available.
{
{ Let the torrent of replies, suggestions, comments, abuse, gossip,
{humour, money, beer, etc. flow (pun intended) ...
{
{Best wishes to all,
{
{"Zip".
{>>>--->
{
{UTSWMC, Dallas, TX.
{
{
{
{
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