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Your best bet in my view is the dye carboxyfluorescein succinimidyl ester;
Do a line search for Parish, C. He has used it best (and I believe first).
It has all the attributes you need, and excites at 488nm. You can even
enumerate proliferation generations with it.
Regards,
Nick
We intend to perform an experiment which will involve the labelling of
isolated mouse tumour cells with a fluorescent dye, prior to introducing
these cells into a mixed population. The idea is to be able to detect these
original cells in the mixture by flow, after a period of time lasting around
two hours. So, the question is this: which dye would any of you esteemed flow
gurus recommend for this, which will:-
Readily be taken up by viable cells.
Not be removed by washing immediately afterwards.
Persist for at least two hours, without serious loss of fluorescence.
Potential excitation wavelength for the dye to be used is not a
problem, as the analysis will be performed using a FACStar+ with a choice of
488nm., UV & dye laser excitation available.
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