Request to Join CYTOMETRY from the web server
sstaylor@warren.med.harvard.edu
Sat, 20 Jul 1996 18:32:20 GMT
I would like to try a 3 colour experiment. The aim is to study the
effect of transfected constructs on cell cycle progression. So I need
to gate for transfected cells so I am cotransfecting a gfp construct
although I could us a CD2 construct. I need a DNA stain for cell cycle
analysis and I want to do an Ab labelling as well. Options I have come
up with so far are 1) gfp(transfected cells); PI (DNA) and Cy5(Ab); or
2)Cy3-CD2 (transfected cells) ; FITC (Ab) and Hoechst (DNA). Does
transfection of gfp, or mere transfection alone alter cell cycle
profiles? Any advice would be appreciated.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
