Megakaryocytes

(no name) ((no email))
19 Jul 96 9:46:27 EDT

We have just begun a pilot study with TPO in breast-Ca and are currently using
a three color panel of CD41FITC(BD), CD90PE(PharMingen) and CD34PerCP(BD) to
define the mega. The major problem is to be sure that you are not identifying
platelets attached to cells. I would recommed the use of ACD-A as an
anticoagulant to reduce the 'platelet stickiness'; and you might want to
include CD62 for activated platelets and a marker to ensure that the CD41+
are not attached to monocytes or granulocytes. We are using a 50,000 event
live gate in the lowSSC and the majority of the CD41+CD34+THYdim cells are in a
region just above the lymphocytes and in our normal GCSF stimulated donors this
represents approx 0.06% total cells.
Good Luck ,
April

April G. Durett
Technology Transfer- Flow Cytometry (R8.1421)
Blood-Marrow Transplantation (Box 024)
The University of Texas MD Anderson Cancer Center
1515 Holcombe Blvd
Houston TX 77030
Lab/Voice (713)7924367
FAX (713)794-4902


Home Page Table of Contents Sponsors Web Sites
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu