Re: Chromosome sorting

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Dr. Sharp,

Though I believe it is possible to obtain good chromosome
resolution when exciting with the 458 nm laser first, I would not
recommended it.

I prefer triggering on UV excited fluorescence since it is brighter
(usually 2-4X) and exhibits better chromosome peak resolution than
fluorescence that results from excitation at 458 nm.

=====================================================================
John J. Fawcett, fawcett@lanl.gov
Life Sciences Division, LS-3 505/667-9633 : office(vmail)
Los Alamos National Laboratory 505/667-2750 : LS Business Unit
P.O. Box 1663, ms M888 505/665-3024 : FAX
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USA

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu