DNAse and TdT apoptosis assay

Jeffrey A. Clapper (clap@iastate.edu)
Mon, 15 Jul 1996 12:03:07 -0500

Greetings:

We have a client who wants to generate a postive control for the TdT
apoptosis assay by treating normal cells with DNAse. I have a few questions
for anyone who has done this.

1. Which type of DNAse would best mimic nucleosomal cleavage?

2. Is it correct to assume that the DNAse treatment should be done after the
cells have been fixed in formaldehyde and ethanol?

3. What concentration, duration, temperature, and buffer should be used?

4. Does it work?

Thanks in advance.

Jeff Clapper
Cell and Hybridoma Facility
Iowa State Univeristy
515-294-8504

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