D cyclins

Michael Fox (MFox@vines.ColoState.EDU)
Mon, 15 Jul 96 9:26:31 MDT

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Does anyone out there have experience measuring D cyclins in CHO cells? I am
trying to measure cyclin D1 in CHO cells using a Pharmingen human antibody
that should cross react with mouse and hamster, but we get no staining of the
cells. Do CHO cells express cyclin D1 (or other D cyclins)? Does anyone
know of another source of antibodies against D cyclins that have been tested
against hamster cells?

Thanks for any input.

Mike Fox
Colorado State University

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu