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It seems CNFDA was available from molecular probes a few years ago
but it is listed as "to be discontinued when our current stocks are
depleted" in ed 5 (1992-4) of the handbook - and not listed at all in
the 1995/6 UK price list.
Others mentioned TO-PRO-3, (and TO-TO-3). I've tried TO-PRO-3 with
yeast and the results are certainly believable. I am a bit concerned
about stories that it may enter viable cells too. I analysed my
samples immediately after adding the dye and didn't detect a problem
with staining of viable cells - although in the flow world it isn't
always easy to analyse immediately ;-)
Another suggestion was carboxy SNARF-1 AM acetate ("a red excitable
pH indicator that is only cleaved to fluorescent SNARF by live
cells"). I haven't followed this lead any further yet.
DiTBAC4(5) was mentioned here on the list.
More than half of the responses were asking for this information so I
would assume that there is a lot of interest in red excitable dyes
(hint to any dye manufacturers out there!!!).
I'll also point any interested parties to the URL
http://pcfcfh.dbs.aber.ac.uk/aberinst/mcytmain.htm#7
where I have begun to compile a list of 633-excitable dyes.
Many thanks again,
Haze
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| Dr Hazel Marie Davey hlr@aber.ac.uk |
|Sefydliad y Gwyddorau Biolegol*Inst. Biological Sciences|
|Prifysgol Cymru * University of Wales|
| ABERYSTWYTH, Ceredigion, CYMRU / WALES SY23 3DA |
| http://pcfcfh.dbs.aber.ac.uk/index.htm |
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