Assay for Tissue factor on monocytes
Brian Rabinovich (brian.rabinovich@utoronto.ca)
Sun, 7 Jul 1996 19:13:20 -0400
I am trying to set up an assay for essessing TF expression on human
monocytes as a determinant of PCA. I will be obtaining my PBMC from whole
blood (probably sodium heparinized). I am wondering if anyone has
experience in this area and could help me set up the protocol? I am
thinking of stimulating the PBMC in OPTI-MEM/antibiotics/10%FCS/1X
B-mercaptoethanol with PMA and ionomycin for four hours prior to staing. Is
this appropriate? Should I block protein synthesis with Brefeldin A and
stain for intracellular TF as well? Any help would be appreciated. Thanks
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
