Re: Phagocytosis by Flow
Jeffrey A. Clapper (clap@iastate.edu)
Tue, 25 Jun 1996 12:11:02 -0500
>From: "JOAN CUNNICK" <jcunnick@iastate.edu>
>Organization: Iowa State University
>To: "Jeffrey A. Clapper" <clap@iastate.edu>
>Date: Tue, 25 Jun 1996 10:46:22 GMT-600
>Subject: Re: Phagocytosis by Flow
>Priority: normal
>
>Jeff: please pass this information on to others.
>
>Phagocytosis on the Flow cytometer can easily be performed by using a
>Phagotest Kit from Orpegen in Germany (Med.-Molekularbiologische
>Forschungsgesellschaft, mbH, Czerny-Ring 22, 6900 Heidelberg, Tel
>06221/9105-0, Fax 06221/910510
>
>I have used this kit equally well for human, swine, and rat blood
>samples. It supplies FITC-conjugated E.coli that are opsonized along
>with a quench solution (a blue reagent-trypan blue?) to quench the
>FITC of bacteria binding to the outside of phagocytes. Also included
>are a lysing solution to get rid of RBC when using blood samples, a
>wash/fixative, and PI solution to distinguish prokaryotes and
>eukaryotes and eukaryote aggregates. The major problem I have had
>with the kit is the expense of purchasing it in Duestch marcs, and
>paying for shipping and customs charges to get into the USA. I do not
>believe that other companys in the US sell this kit or market it.
>
>Conversely, there is a protocol in Current Protocols in Immunology
>for the FITC conjugation of bacteria. This protocol uses a
>fluorescent microscope. It should also work with a flow cytometer if
>the phagocytes are in a single cell suspension.
>
>Joan E. Cunnick; Microbiology, Immunology, and Preventive
>Medicine (MIPM); 207 Science I; Iowa State University; Ames, IA 50011
>phone:515-294-2070 FAX:515-294-6019 e-mail:jcunnick@iastate.edu
>
>From here to there, from there to here, funny things are everywhere!
>
Jeff Clapper
Cell and Hybridoma Facility
Iowa State Univeristy
515-294-8504
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
