cytometry of adipocytes or sebocytes

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our work involves differentiation mechanisms of sebocytes, whose primary
differentiation phenotype is lipid accumulation. we are trying to use nile
red fluorescence to ascertain the level of lipid accumulation in cultured
cells, but are getting FACScan results that leave much to be desired in
their consistency.
i am looking for any suggestions?
thanks very much,
alex kentsis.

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu