Re: WHAT DOES LOG MEAN ON XL COULTER

Gerhard Nebe-von-Caron (Gerhard.Nebe-von-Caron@unilever.com)
10 Jun 96 10:34:58 Z

What software version do you run? With our system (1.5/1.22
whatever that means) you can even compare the lin and the
log peak channels if you aquire both as they are not created
by seperate amplifiers in the XL.
Means values are always slightly different, but how to
caculate means in log-space (and why people do it if the
cluster is not a log normal distribution) remains a
philosophical question.

Gerhard Nebe-v.Caron
Unilever Research, Colworth Laboratory
Sharnbrook, Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
E.mail: gerhard.nebe-von-caron@unilever.com


______________________________ Reply Separator _________________________________
Subject: WHAT DOES LOG MEAN ON XL COULTER
Author: tkute@bgsm.edu at INTERNET
Date: 08/06/96 00:45

I am having a hard time getting an answer on what exactly the log
scale means on the Coulter XL instrument. When I gate on the peak of
interest, the Mean X value has no relationship to the value that is
observed in the histogram. How would one try to compare to peaks of
different fluorescent units?
Thanks in advance for any help.

Dr. Tim Kute email: tkute@bgsm.edu
Associate Professor in Pathology Tel#: 910-716-2950
Wake Forest Univ-Bowman Gray School of Medicine Fax#:910-716-7595
Medical Center Blvd
Winston-Salem, NC 27157-1072

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