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To explain:-
A couple of months ago, while processing Thiazole Orange retics on our cytometer
an homozygous Hgb E patient's UNSTAINED reticulocyte control (i.e. Rbc's in PBS
alone) showed a very high degree of fluorescence when compared to a normal
unstained control - around 50 % positivity extending over one decade.
This was confirmed recently when the patient returned for a follow up visit.
As this is the only E disease patient we have, since it is rare in the middle
east, we were unable to establish whether this is due to the Hgb E or to some
other factor.
Any input from people working in the far east, or input from anyone with ideas
as to what could cause this, would be much appreciated.
Wal Sharp
SQU
Oman
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