Platelets activation

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Dear Fellow Cytometrists:

We are running a leukapheresis (LP) blood assays on the flow cytometer and in
some cases we encounter platelets activation problems where we are unable to
gate them out especially if they cross link WBCs.

What are the different procedures to stop platelets from being activated
during handling?

Osama Nabulsi
Amgen Inc.
(805)447-1594

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu