Calcein-AM vs CFDA

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Dear Fellow Cytometrists:

I have been adviced by Molecular Probes Inc. to use Calcein-AM instead of
5-Carboxyfluorecein diacetate for staining for live cells.

Have anyone used any of those dyes especially on attached cell lines? Thanks
in advance for any responses.

Osama Nabulsi
Amgen Inc.
(805)447-1594

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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu