FMC-7 and platelet activation
ctnebe (ctnebe@t-online.de)
Thu, 30 May 96 01:37 +0100
FMC-7 has been shown of good value for B-NHL classification. It occurs in
soluble form in plasma. So you need 2 prewashings and a mouse serum blocking
step to prevent capture of your ab and unspecific binding on eg. T cells. By
this method you will obtain reliable results. Unfortunately the clone is lonely
and has no CD designation. CD22 behaves similar but still different to FMC-7.
Therefore we stick to it esp. for dissecting CLL, ln positive CLL and other
lymphomas.
It is possible to fix platelets for the analysis of activation antigens on
their surface. There are at least three mixtures out. Two are commercially
available from Sarstedt using their syringe system. They supply two types for
plt activation, one fixes and the second stabilizes for further activation in
vitro like ADP stimulation. They are based on work from Dr. Tschoepe at the
diabetes research institute at Duesseldorf in Germany. For the analysis of
intracellular cytokines and activation antigens in platelets please check the
very recent paper from Sedlmayr in cytometry.
C. Thomas Nebe
Klinikum Mannheim, Med. Faculty University of Heidelberg, D-68135 Mannheim
ctnebe@t-online.de
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
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If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
