Re: 7-AAD with fixation

Dave Coder (dave@nucleus.immunol.washington.edu)
Tue, 28 May 96 15:17:36 -0700

Several people have asked about the use of fixatives with 7-AAD detection of
non-viable cells as described by T. Fetterhoff et al. 1993. I've added a
basic protocol for this to the page that describes the general 7-AAD
procedure:

http://nucleus.immunol.washington.edu/Research_facilities/Apps/7aad.html

Click on the Fetterhoff reference for details.

Dave Coder

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David M. Coder, Ph.D.
Director, Cell Analysis Facility
http://nucleus.immunol.washington.edu/Research_facilities/cell_analysis.html

Editor, ISAC WWW Home Page
http://nucleus.immunol.washington.edu/ISAC.html

tel. 206-685-3014
fax. 206-543-3480
e-mail: dcoder@u.washington.edu

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