help for intracellular cytokines staining

nuti (nuti@iris02.biocine.it)
Mon, 27 May 1996 10:00:59 +0200

We are interested in performing intracellular cytokines staining by using a
flow cytometric system on frozen lymphocytes.
Question: during the freezing step do variations or alterations in the amount
of cytokines occur?
Thanks for your help !

SANDRA NUTI and M.GRAZIA PERARI


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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu