Re: Excluding non-viable cells
Dave Coder (dave@nucleus.immunol.washington.edu)
Wed, 22 May 96 12:18:45 -0700
Works great. For details see the entry in my lab's web site. Correction of
fluorescence overlap between 7-AAD and PE is important. Look at the color
illustrations that accompany the procedure.
http://nucleus.immunol.washington.edu/Research_facilities/Apps/7aad.html
Dave Coder
Begin forwarded message:
Date: Thu, 9 May 1996 21:46:49 -1000
To: cyto-inbox
Subject: Excluding non-viable cells
From: "Steven T. Komura" <tetsujin@hula.net>
X-Mailer: PROCOMM PLUS 3.0 for Windows
Anyone have any experience using 7-AAD to gate out non-viable cells in
a two-color system (PE or RD1 in combination with FITC) using a 488 nm
laser (Epics Profile II)?
Steven T. Komura tetsujin@hula.net
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