Red tracking dyes for yeast?

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We wish to label yeast cells using a red or orange dye. The
properties required are that it should no not diffuse from labelled
to unlabelled cells and should be retained for many hours within
stained cells. Ideally the labelling would be bright enough that
cells would remain detectably labelled even after dilution over several generations.

Has anyone had success using PKH26 (Zynaxis/Sigma) for labelling yeast cells?
Or how about CMTMR CellTracker orange (Molecular Probes)?
Or even MitoTracker red (Mol Prob)?

Thanks for your help

Dan
_______________________________________________

Dr Dan Deere (ddeere@rna.bio.mq.edu.au)
Research Fellow
Australian Environmental Flow Cytometry Group
School of Biological Sciences,
Macquarie University,
Sydney,
Australia NSW 2109.
Tel- +61 2 850 8150/8238 or 8685510
Fax- +61 2 850 8245
_______________________________________________

• Next message: Edward Srour: "Re: MRK16 source"
• Previous message: Margaret Cooley: "EPICS XL data storage on CD's"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu