SV: Kappa and Lambda

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Fra: owner-cyto-sendout
Til: Cytometry Mailing List
Emne: Kappa and Lambda
Dato: 16. mai 1996 09:57

Dear Flow-ers,

This is another basic kind of question ... but as usual I'm sure that I'm
not the only one who has trouble with this.

My staining for kappa/lambda expression is very disappointing - separation
of negative and positive populations is miserable and despite using CD45vSS
to selectively gate lymphoid cells, I still get 2-3% dual positive events
(using color backgating these dual positives appear to be occasional stray
granulocytes/monocytes.)

I'm wondering what the consensus is for clinical samples ie: kappa/lambda
dual labels or do people use kappa/CD19 and Lambda/CD19 (I doubt whether
this is necessary since I use CD45/SS as a primary gating strategy but I
could be wrong!!)

Also what brands of Kappa/Lambda antibodies have people found to be most
successful - I currently use BD.

As always, any suggestions greeted with many thanks.

Peter Chapple

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