Re[2]: Sorting G0 cells? -Reply

Gerhard Nebe-von-Caron (Gerhard.Nebe-von-Caron@unilever.com)
20 May 96 10:07:02 Z

See also :
Ken Toba et al.: Simultaneous 3 color analysis of surface
phenotype and DNA-RNA quantitation using 7AAD and pyronin Y
J.Immunil. Methods 1995 182:193-207.

Apart from the nice trick of blocking the pY DNA binding
with the 7AAD they also measured cd71 in that mix.

Gerhard Nebe-v.Caron
Unilever Research, Colworth Lab.
Sharnbrook, Beds. GB-MK44 1LQ
Tel.:+44(0)1234 222066
Fax.: 222344
Gerhard.Nebe-von-Caron@unilever.com

______________________________ Reply Separator _________________________________
Subject: Re: Sorting G0 cells? -Reply
Author: hms@shapirolab.com at INTERNET
Date: 17/05/96 00:37

I second David Hedley's nomination of the transferrin receptor (CD71) as
probably discriminating G0 and G1/S/G2/M phase cells, at least in
lymphocytes; we also looked at that (when it was OKT9) years ago, and the
advantage of the transferrin receptor is that it is presumably expressed on
most if not all proliferating (i.e. G1/S/G2/M) cells, not just lymphs. We
should shortly have some harder data from stimulated lymphocytes in which we
will measure CD3, CD4 or CD8, CD71, and DNA and RNA, once we get the
appropriate antibody labels.
-Howard


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