Sorting G0 cells. Reply
Edward Srour (edward_srour@iucc.iupui.edu)
Thu, 16 May 1996 17:01:25 +0800 (U)
As mentioned by Howard Shapiro, Hoechst and Pyronin Y can be used to sort
cells in G0. Also as mentioned, PY is toxic. We have successfully used both
dyes to sort VIABLE cells in G0. We have only published this in abstract form
and the paper is submitted. it is a little tricky, but doable. We use PY at
3 ug/mL. PY at such low conc. may stain mitochondria only, and not RNA so one
has to verify that such a conc. is staining RNA. We find that with different
cells (human versus murine) staining and toxicity varies. Also cells have to
remain as much as possible in the dark, especially after sorting. The fact
that you want G0 cells which should have the least amount of PY in them helps.
Usually G1 cells or G2 and M cells do not do as well.
Edward F. Srour
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