TUNEL, biotin-dUTP, and flow cytometry
Dr. Howard Petrie (h-petrie@ski.mskcc.org)
Tue, 28 Nov 1995 20:12:29 -0400
Dear Fellow Cytometrists: We are about to start performing DNA strand
break analysis using TDT and biotinylated dUTP. I am wondering whether
anyone has compared Promega's biotin-21-dUTP vs. Boehringer's
biotin-16-dUTP, or can reliably guess whether either should work better. I
would guess that a 16 spacer should be sufficient, but maybe not... Also,
I wonder if anyone could explain the rationale as to why uridine is used
rather than a more conventional DNA nucleotide? Thanks for your
suggestions.
Howard Petrie
Memorial Sloan-Kettering Cancer Center
Box 341, 1275 York Avenue
New York, NY 10021
h-petrie@ski.mskcc.org
(212)639-2149
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
