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You did not specify what types of cells you were having the clumping
problems with. You might try filtering the cells through nylon mesh (we
buy ours from Tetko). This works well for cell suspensions made from mouse
spleen, thymus, and epidermis. I don't know if thiomerosal is a good thing
to include in staining buffer for flow cytometry. Sodium azide (0.02% to
0.1% depending on who you talk to) is often added to staining buffer to
prevent capping. I hope this helps.
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Ifor Williams, M.D., Ph.D. | E-mail #1: iforw@delphi.com
Assistant Professor | E-mail #2: irwilliams@bics.bwh.harvard.edu
Division of Dermatology |
Brigham and Women's Hospital |
75 Francis St. |
Boston, MA 02115 |
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Paul Hallberg recently posted this message:
>Hi Fellow Flow Experts !,
>
>Does anyone know of a stain(besides "PI") or an antibody, preferably FITC
>conjugated, to detect cell viability? (Please include any vendors if known).
>Also is there any buffer or component that can be added to prevent clumping
>of cells during a cell staining incubation?
>Currently, I stain in PBS(1x) with 0.1% BSA and 0.01% thimerasol for 1 hr. on ice.
>Any suggesstions would be appreciated.
>
> Paul L. Hallberg
> HMJF/Retrovirology
> Rockville, MD
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