cell viability via flow

PHALLBERG (phallberg@hiv.hjf.org)
Thu, 16 Nov 1995 16:35:03 -0500

Hi Fellow Flow Experts !,

Does anyone know of a stain(besides "PI") or an antibody, preferably FITC
conjugated, to detect cell viability? (Please include any vendors if known).

Also is there any buffer or component that can be added to prevent clumping of
cells during a cell staining incubation?

Currently, I stain in PBS(1x) with 0.1% BSA and 0.01% thimerasol for 1 hr. on
ice.

Any suggesstions would be appreciated.

Paul L. Hallberg
HMJF/Retrovirology
Rockville, MD

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