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An interesting phenomenon that we initially noticed with fluorescent
microscopy and later confirmed with flow cytometry is the presence of a
population which stains a color that has properties of both live and dead
stains. We find that as cell cultures are stressed (chemical, heat,
radiation) the level of these 'intermediate' colored cells increases. We
have found that there seems to be a correlation between the relative amounts
of stress and the population of intermediate colored cells.
This has been shown with a number of different genera and species. There does
seem to be some differences in the amount of the intermediate population with
different cultures, but their presence seems to be universal.
If there are other researchers out there doing work like this and can offer
any theories or explanations for this phenomenon, we would very interested in
hearing from you.
Bill Rutherford (Rutherfor@phibred.com)
Beth Harman (Harmane@phibred.com)
Lyse Norian (Norianla@phibred.com)
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