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>
>
> Hello Flowers!
>
> Does anyone have experience with surface CD38/cytoplasmic kappa/lambda?
> I would like to find out the simplest procedure.
>
> Thanks a lot in advance.
>
> Mike
>
Hello to all:
Surface CD38/cytoplasmic kappa/lambda works nicely with the
Fix&Perm reagents provided by An der Grub, Vienna. The only
critical step is the careful washing of the bone marrow samples
(3x) before adding the fixation reagent A. This is necessary
to avoid artefactual clonality of plasma cells in presence
of a large excess of clonal light chains in plasma. After
washing cells are incubated with the permeabilizing reagent B
and antibodies. We have good experience with FITC and R-PE
anti-light chain reagents from DAKO and a R-PE/Cy5-tandem
CD38 from CALTAG. Other companies may provide good reagents
as well.
A discrimination between surface and cytoplasmic CD38 which
would depend on preincubation with the CD38 monoclonal
before permeabilizing is not necessary for the identification
of plasma cells.
Gregor Rothe
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Institute for Clinical Chemistry and Laboratory Medicine
University of Regensburg
D-93042 Regensburg, F.R. Germany
Tel. +49 (941) 944-6208, or -6233
Fax +49 (941) 944-6202
Internet: Gregor.Rothe@KLINIK.UNI-REGENSBURG.DE
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