G2 synchronization
ZBIGNIEW DARZYNKIEWICZ (darzynk@nymc.edu)
Wed, 15 Nov 1995 10:58:35 -0500
To the ongoing discussion about obtaining cells synchronized in the
cycle I would like to add the following warning: The synchronization
by transient cell arrest in the cycle induces growth imbalance and
dramatically alters expression of cyclins and other cell cycle
regulatory proteins. For example after double thymidine block we have
observed "unscheduled" expression of cyclins B1 and A in cells at the
G1/S boundary, over five-fold increase in expression of cyclin E, and
40% increased total protein content [Gong et al., Cell Growth &
Differentiation, 6: (November issue) 1485-93, 1995]. Kinetic and
metabolic properties of so synchronized cells are much different
compared to the cells from asynchronous, exponentially growing
cultures.
Zbigniew Darzynkiewicz
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