Re: sorting of G2 cells

MORGANTL@aol.com
Tue, 14 Nov 1995 00:31:31 -0500

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Maybe in reply to: kweber@vs4.im.med.umich.edu: "sorting of G2 cells"

Sorting or isolating G2/M cells is one of the most difficult and vexing
problems in cell biology. Sorting by FSC (size) works poorly or not at all.
Most people in my field (radiobiology) use mitotic shake off, double
thymidine block or centrifugal elutriation to collect cells in G1 or at the
G1/S border and then allow the cells to progress normally into G2. This
progression can be followed easily with flow cytometry and almost certainly
results in a high percentage of viable cells.

Next message: SKELLEY@flowcyt.cyto.purdue.edu: "Linscott's"
Previous message: Bruce Davis, MD - Clinical Pathology: "Re: CD38/kappa-lambda"
Maybe in reply to: kweber@vs4.im.med.umich.edu: "sorting of G2 cells"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu