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Date: Sat, 11 Nov 1995 16:42:13 +0100 (MET)
From: Bjarne Moeller <bkm@biobase.dk>
Subject: HIV viral load and flow cytometry: methods & perspectives?
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=B7 To the best of my knowledge I have not seen any longitudinal data tha=
t has =
validated the number of infected cells with clinical outcome. We have do=
ne =
something a little bit different than what was originally published by =
Schmittman at the NIH when attempting to quantitate the number of infecte=
d cells =
in HIV. He used limiting dilution's followed by PCR for the gag products=
=2E In =
our approach we sorted cells into dilution's starting at 10K and ending a=
t 10 =
cells followed by PCR for the gag products. These were measured by a tec=
hnique =
known as the liquid hybridization protection assay which is a qualitative=
assay =
for the presence of these products. These results were only studied on a=
cross-
sectional basis and were only intended to answer questions related to oth=
er =
infected cell types such as monocytes. However, I wide range of results=
were =
seen, some positive at <100 cells and other still negative at 10,000 cel=
ls. =
This might lead one to believe a correlation might exist with clinical ou=
tcome.
Stephen P. Perfetto
Department of HIV Disease Prevention
Walter Reed Army Institute of Research
1600 East Gide Drive
Rockville, MD. 20850
_________________________________________________________________________=
______
Subject: HIV viral load and flow cytometry: methods & perspectives?
From: Bjarne Moeller <bkm@biobase.dk> at Internet_Gateway
Date: 11/11/95 4:42 PM
The clinicians at our center has confronted me with a few relatively =
simple questions, which I am not able to answer and therefore has =
forwarded to the List of Flow'ers:
We have been wondering if anybody has used flow cytometry to count the =
number of infected cells in HIV-1 patients. Any communications of =
correlation to molecular biology (ie. semiquantitative HIV-1 RNA-PCR)?
If so, is the infected fraction predictive of clinical outcome of HIV-1 =
infected patients? =
_________________________________________________________________
Bjarne K. Moeller, MD =
Dept. of Clin. Immunology Voice: +45 8949 5304
University Hospital of Aarhus Fax: +45 8949 6007
Skejby Sygehus =
DK-8200 Aarhus, Denmark E-mail: bkm@biobase.dk
_________________________________________________________________
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