Re: Beyond gating revisited
Bruce Milthorpe (brucem@cbme.unsw.edu.au)
Fri, 10 Nov 1995 12:40:07 +1000
Not that I gate lymphocytes or such, but about non-specific binding...
Really non-specific binding is that part of the 'binding' or adsorption
or whatever that gives a signal that is linearly proportional to
concentration as far as we can determine. Specfic binding is associated
with a set number of sites (and binding constant[s]) which gives an
assymptotic curve. The physical reasons why are enough to give one a
headache, so maybe that's why Someone invented maths!:-)
Bruce Milthorpe
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
