RE: fixative/HIV+samples/Autolyse
kukuruga%kasle1.dnet.wayne.edu@rocdec.roc.wayne.edu
Fri, 10 Nov 1995 09:44:27 -0500
Specifically in response to 'Jane'...many of the commercial lysing reagents
have paraformaldehyde already in them, so certainly you should be able to
add it to yours. You should test it, though, to be sure the results are
not changed. (Question...are you certain your BD lysing agent DOESN'T have
a fixative in it?)
General comment on paraformalddehyde...I've used histo grade formalin for
years as an alternative to paraformaldehyde, with good results. Parallel
samples fixed with either paraf. or form. yielded similar results; formalin
preserved size paramenters and morphology as well. Plus...it's cheaper,
stable, easier and not as toxic to handle.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
