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A special concern is with CD4 or CD8 blasts that tend (in our
experience) to scatter higher in FS and SS. On the other hand, the CD14
gate is essential to control the CD4 contribution from the monocytes.
I have not yet investigated what we loose in CD4(bright) and CD8
using the B-D CD14/45 control; but I think I will in the future.
Denis Snider
McMaster University
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On 4 Nov 1995, Walter Sharp wrote:
> Does anyone else out there feel that the current widely held concept of
> tightening Lymphocyte gates/bitmaps until Monos are excluded (CD14/45) is a bit
> like a catch 22 situation ?
>
> The premise is that lymphocyte subsets are evenly distributed throughout the
> lymphocyte population as isolated by FS and SS - my experience is often the
> opposite.
(cut remainder of message)
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