Re: CD34

Noor Parker (Noor.Parker@med.utas.edu.au)
Wed, 25 Oct 1995 15:17:56 +1000

Messages sorted by: [ date ][ thread ][ subject ][ author ]
Next message: Scott R Burger: "CellPro and CD34 enrichment"
Previous message: Dennis_Young@CIS.ucsd.edu: "Re[4]: Query regarding cell cycles"

>Hi,
> How do people out there measure the CD34+ population by flow, do you
>gate on CD45+ cells or around the MNC fractions?
> Let me know.
> Many thanks.
> Ed.

We use the method decribed by sutherland in Experimental Haematology
22:1003-1010(1994). An australian multi-centre consensus study has shown
that the most reproducable results are obtained by this method which uses
both CD45 and CD34 expression in conjunction with scatter properties.

Regards

Noor M. Parker
Noor.Parker@med.utas.edu.au

Next message: Scott R Burger: "CellPro and CD34 enrichment"
Previous message: Dennis_Young@CIS.ucsd.edu: "Re[4]: Query regarding cell cycles"

CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu