Re: Fluorescent insulin
/G=Gerhard/S=Nebe-von-Caron/OU=1890CHPE/O=TMGB.URC/@LANGATE.gb.sprint.com
Tue, 24 Oct 1995 05:14:00 -0400
That reminds me of good old Burghard Bohn from Heidelberg,
who introduced the idea of "receptor-ligand" binding to the
field of flow cytometry in the late 70's. He used the
insulin receptor as a model for endicronological studies
possible by FCM. They simply labelled it with FITC. I have
only got his article "Flow Cytometry: a novel approach tfor
the quantitative analysis of receptor-ligand interactions on
surfaces of living cells", Molecular and Cellular
Endicronology, 20 (1980) 1-15. I assume he published a few
more papers on that.
Gerhard Nebe-v.Caron
Unilever Research, Colworth Laboratory Sharnbrook,
Bedfordshire
GB - MK44 1LQ
Tel: +44(0)1234-222066
FAX: +44(0)1234-222344
E.mail: gerhard.nebe-von-caron@urcgb.sprint.com
______________________________ Reply Separator _________________________________
Subject: Fluorescent insulin
Author: Dean.Hewish@mel.dbe.csiro.au at INTERNET
Date: 23/10/95 21:23
A colleague asked me to enquire about fluorescently labelled insulin.
Any fluorochrome would do.
Has anyone used such a reagent for receptor binding studies on insulin
responsive cells (not necessarily by flow)?
If so, did it show reasonable binding and how was it labelled?
Does anyone know of a commercial supply, or do you need to make your own?
Thanks,
Dean R. Hewish, Cell Biologist & Flow Cytometrist. ( and Amateur Malacologist)
CSIRO Biomolecular Engineering, 343 Royal Parade, Parkville, 3052,
Victoria Australia.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
