Re: Right angle scatter with UV laser line?

Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Thu, 12 Oct 1995 08:53:15 +1100

At 11:06 AM 11/10/95 -0500, Raymond B. Hester wrote:
>
>We don't do that much UV on our flow but I've just across what seems to
>be a problem and I knew someone out there could help.
>
>Do you not get right angle light scatter (or very little of it) when
>using the UV line? I was just setting up for an experiment in which we
>will be using UV on our FACS 440. For alignment, I was using some old
>Polyscience beads
>(about 5 micron dia.) that still fluoresce and I didn't see much side
>scatter.

Two things spring to mind:
When I did some UV work on a FACS 440 some years ago, I found the side
scatter to be MUCH lower sensitivity than with visible laser; I think that
UV light is not scattered as much, and the PMT was less sensitive to UV.

I did not get ANY UV side scatter until I removed the 488 bandpass filter
from the side scatter detector! (took me at least a week to think of it...)

Good Luck, Joseph.

Joseph Webster (O.I.C. Flow Cytometry & Communications) ===
Centenary Institute of cancer Medicine & Cell Biology / \
Locked Bag No.6 || o o ||
Newtown, NSW 2042 || | ||
AUSTRALIA. | \___/ |
Ph: 61-2-565-6110 \ /
Fax: 61-2-565-6101 |||||
E-Mail: J.Webster@centenary.usyd.edu.au

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