no subject (file transmission)
Tom Mchugh (mchugh@pangloss.ucsf.EDU)
Wed, 11 Oct 1995 12:56:16 -0700 (PDT)
Regarding the question about detecting myeloperoxidase in cells by flow:
We detect MPO using anti-MPO-FITC from DAKO, Carpenteria, CA with the
fixation/permeabilization reagent from Pinnacle Biosystems, Burlingame,
CA. We use it on blood and bone marrow and the antibody and reagent
seems to work fine.
We use a number of other antibodies from DAKO with this
fixation/permeabilization reagent and they
work fine (anti-CD3, anti-kappa, anti-lambda, anti-PCNA, etc.)
We however almost always start with ficolled cells from blood or bone
marrow. Some whole blood studies are done after rbc lysis.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
