RE: Dual staining with Hoescht 33342 and PI
kukuruga%kasle1.dnet@rocdec.roc.wayne.edu
Mon, 9 Oct 1995 13:02:52 -0400
Two possibilities which may contribute to your efffect: 1) PI does absorb/
excite well in the UV, therefore emitting significcant fluorescence from the
UV laser; 2) PI will quench the H33342 signal.
CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories
and distributed free of charge as an educational service to the cytometry community.
If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL,
Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu
EMAIL robinson@flowcyt.cyto.purdue.edu
