 |
 |
 |
 |
I have clients who plan to do DNA content and P53 on human
leukocytes. Easy, right? The catch is, they will need (for reasons
unclear to me) to store the separated leuks for a long time
(possibly months) before we analyze the samples. Can anyone suggest
a method with a decent chance of success? At the moment we're
considering freezing in PBS (or HBSS) + 10% DMSO--for some reason they
seem to think they won't be able to take sterile media with them
(they'll be out of the country, but apparently will have access to
cryogenic freezers.) Can they wait till the samples get back here to
label the P53, or should that be done at the time of collection? All
the methods of fixation I'm familiar with seem best suited to the
clinical setting, where storage is generally on the order of days,
not months. Are they dreaming?
Steve
*********************************************************
Steve G. Hilliard Life's too short
Cell Analysis Facility to drink bad beer!
University of Georgia
|
|
|
|