sorting insect cells

Joseph Trotter (trotter@scripps.edu)
Mon, 18 Sep 95 13:49:21 PDT

Hello,

Anyone have any words of wisdom on optimizing conditions for the
sorting of cell surface stained drosophila cells? By the time we see the stained
cells a large percentage are "dead". Anybody have any tricks for keeping them
a little more happy during preparation (currently at room temp) and any
subsequent sorting?

Thanks,

Joe


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