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Title: Recent advances of flow cytometry in fundamental and applied
microbiology
Author: Fouchet, Pierre; Jayat, Chantal; Hechard, Yan; Ratinaud, Marie Helene;
Frelat, Gerard
Source: Biol. Cell 1993 78 01/02/95 95-109
Year: 1993
Country: Fontenay-aux-Roses, 92265, Fr.
Abstract A review with 215 refs. focusing on recent applications of flow
cytometry (FCM) in microbiol. research (1987-mid 1992). It tries to give a
scope of the important breakthroughs which occurred in this field during this
period. The tech. difficulties of microorganism anal. by flow cytometry is
briefly appraised. The significance and the limits of the different microbial
cell parameters attainable by flow analyses are systematically evaluated: light
scatter for cell size and structure, fluorescence measurements for
quantification of cellular components, microbial antigen detection and cell
physiol. activity estn. Emphasis is given on the new technol. advances which
appeared in the last two years. The second part of the review is devoted to the
anal. of the usefulness of flow cytometric approach in the different fields of
microbiol.: fundamental studies in microbial physiol., differentiation,
microbial ecol. and aquatic sciences, medical microbiol., parasitol., microbial
pharmacol. and biotechnol.
______________________________ Reply Separator _________________________________
Subject: Future prospects
Author: hlr@aber.ac.uk at INTERNET
Date: 12/09/95 22:02
We are preparing a review on various aspects of flow cytometry in microbiology
and like all good reviewers we will include a "future prospects" section. We
have ideas of our own for this, but would also be pleased to hear from anyone
else about how they would like flow cytometers to develop to make them more
useful for the microbiologist.
Do you currently find the sensitivity of your flow cytometer limiting? Are
there flow cytometric techniques that should but don't work for microbes? Does
the future lie in better instrumentation or in better dyes? Please remember to
include in any correspondence the make and model of flow cytometer that you
use.
If you would like to offer your opinions then you can Email me on:
hlr@aber.ac.uk
or if you prefer you can fill in the feedback form on my website:
http://pcfcfh.dbs.aber.ac.uk/
If anyone else expresses an interest in this information then I will Email a
summary to the list or to interested individuals. If you want your views to
remain anonymous please let me know.
Of course any very recent (1995) (p)reprints in this field that you think we
should include would also be most welcome.
Snail address:
Dr Hazel M. Davey,
Institute of Biological Sciences,
Edward Llwyd Building,
University of Wales,
Aberystwyth,
SY23 3DA
WALES, U.K.
Many thanks in advance for your help,
Hazel
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| Dr. Hazel Davey (hlr@aber.ac.uk), Univ. Wales, Aberystwyth, UK, SY23 3DA |
| WWW : Flow Cytometry | Welsh | Brewing : http://144.124.112.37/index.htm |
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