working with live trypanosomes
system@flocyt.int-med.uiowa.edu
Fri, 08 Sep 1995 14:14:31 EDT
Greetings,
We have an investigator that wants to study live trypanosomes (the
kind that infect humans) using flow cytometry and eventually sort
based on cell cycle. Has anyone specifically dealt with the biohazards
of live trypanosomes and flow cytometry? Should these little beasts
be considered too hazardous? We sort live human PBL's taking the
"usual" precautions but were not sure what other people's experiences
were with live parasites. The ultimate goal is to extract RNA from
the specific cell cycles after sorting which means using "viable" Hoechst and precludes
fixing with paraformaldehyde. Thanks for any thoughts.
Justin Fishbaugh
University of Iowa
Flow Cytometry Facility
internet: justin-fishbaugh@uiowa.edu
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