Re: FDG for nuclear lac-z?
James W. Jacobberger (jwj@po.cwru.edu)
Sat, 9 Sep 1995 11:31:00 -0400
>
>Has anyone heard of FACS-gal, FDG, Fluoreporter substrate used for lac-z
>localized to the nucleus in live cells? Is this even possible using
>hypotonic treatment?
>
>Just doing a reality check for a flow user.
>
>Thanks,
>Rochelle Diamond
>Caltech Biology
>Flow Cytometry/Cell Sorting Facility
>
Rochelle
We stained cells successfully with FDG that had nuclear localized lac-z.
There were no problems with the standard assay, and using a fixation like
Clevenger's (low formaldehyde/triton X-100), we had better retention than
cells expressing normal lac-z.
Jake
James (Jake) Jacobberger
Associate Professor
216-368-4645 phone
216-368-3432 fax
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