Re: apoptosis

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Usually the apoptotic cells will give a sub-diplolid peak in the
DNA histogram. This is due to extraction of low molecular weight DNA
fragments when the fixed cells are resuspended in PBS for staining. The
efficiency of extraction depends on the cell type and the buffer in which the cells
are resuspended.

There is a large literature on this topic. Search on apoptosis, flow
cytometry and DNA histogram.

Mike Ormerod
34 Wray park Road
Reigate RH2 ODE
UK +44 (0)1737-241726 (voice & FAX)

• Next message: Kevin Holmes: "Protocols for PE Conjugation..."
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CD ROM Vol 2 was produced by staff at the Purdue University Cytometry Laboratories and distributed free of charge as an educational service to the cytometry community. If you have any comments please direct them to Dr. J. Paul Robinson, Professor & Director, PUCL, Purdue University, West Lafayette, IN 47907. Phone:(317) 494-0757; FAX (317) 494-0517; Web http://www.cyto.purdue.edu EMAIL robinson@flowcyt.cyto.purdue.edu