RE: FCS transfer

Eric Martz (emartz@microbio.umass.edu)
Wed, 06 Sep 1995 20:22:10 -0500 (EST)

In message Wed, 06 Sep 1995 12:19:00 -0700, Dennis_Young@cis.ucsd.edu writes:

> We mistakenly transferred a FCS 1.0 file using Fetch 2.1 on the Apple and
> FACSNet on the Host HP in the Automatic mode. This mode saves files as
> text files. Can the FCS data be rescued?

If by 'text' you mean list mode data as ASCII columns of numbers
rather like a spreadsheet (or if you can convert the file to this
form), you could use my A2FCS (ASCII to FCS) program, which is
an improved version of Joe Trotter's TEXT2FCS. A2FCS.EXE is a
DOS program which is part of a test suite not yet fully integrated
into my program MFI (and hence not yet officially released):

ftp://flowcyt.bio.umass.edu/pub/flowcyt/mfi/beta.dir/mfiverif.exe
is the self-unpacking zipped file.

The test suite also includes programs for generating synthetic
flow cytometry 'data' of various sorts.

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