PNA+ Controls

Jonni Moore (moorej@a1.mscf.upenn.edu)
Tue, 05 Sep 95 15:34:01 -0400

We have a user who wants to sort germinal center B cells from human thymus
based on CD20+/peanut agglutinin(PNA)+ criteria. Does anyone know what an
appropriate negative control for the PNA would be. Normal isotype would
not work since it is not an immunoglobulin. We are interested in sorting
both positive and negative populations and need good controls since the
negative population may be <2%.

Thanks for any suggestions anyone might have!
Jonni

Jonni S. Moore, Ph.D.
Director, Cancer Center Flow Cytometry and Cell Sorting Facility
University of Pennsylvania School of Medicine
297 John Morgan Bldg
Philadelphia, PA 19104-6082
Phone: 215-898-6853
FAX: 215-898-4227
e-mail: moorej@a1.mscf.upenn.edu

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