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The reason a whole blood assay is used for immunophenotyping as opposed to
lymphocytes (or other leukocyte populations) separated over density
gradients is to avoid losses in the preparation method, not the lysis
method. Of course, methods in which the red cells are not lysed and there
are no centrifugation steps would be the best to avoid cell loss, but these
are not normally used.
Jan Nicholson
CDC
Atlanta, GA
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Are CD15S (neutrophils) and CD62L (L-selectin) available directly conjugated
to a fluorochrome?
Are there any other surface markers for neutrophils only?
One of the reasons I have heard that people use whole blood when they label
cell surface antigens is so that the do not lose any populations due to
lysis of RBC's. However, don't these procedures all include a lysis step at
the end anyway? Have I missed the point?
Are there other procedures I should know about?
Thanks!
Deb G.
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