Use of Isoton II as sheath fluid

Raymond B. Hester (rhester@jaguar1.usouthal.edu)
Thu, 31 Aug 1995 10:56:49 -0500 (CDT)

As a brief followup on my question about why sorted Salmonella did not
grow when placed back into culture, it looks as if it might have been the
Isoton II.

As suggested by some members of this network, we repeated the experiment,
using PBS as the sheath fluid, and this time the organisms grew when
cultured. This suggests that if anyone else is using Isoton II as a
sheath fluid, they should at least be aware of some of its ingredients:

disodium EDTA 0.38 g/L
sodium fluoride 0.3 g/L
2-phenoxyethanol 3 g/L

Ray

++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
Raymond B. Hester, Ph.D. Someday, after we have mastered the
Flow Cytometry Lab winds, the waves, the tides and
The University of South Alabama gravity, we shall harness the energies
Mobile, AL 36688-0002 of love. Then, for the second time
Email: rhester@jaguar1.usouthal.edu in the history of the world,
Voice: (334) 460-6029 man will have discovered fire.
FAX: (334) 460-6073 -Teilhard de Chardin
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